85-6152-92　Ephrin-A5, Active, Recombinant, Human, hFc-Tag, aa21-203 (EFNA5) 20ug　586876

特徴

• Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.
• Recombinant protein corresponding to aa21-203 from human Ephrin-A5, fused to hFc-Tag at C-terminal, expressed in Mammalian cells.
• Molecular Weight:~50.1kD
• Biological Activity:Measured by its binding ability in a functional ELISA. Immobilized EPHA3(CSB-MP007723HU) at 2ug/ml can bind human EFNA5, the EC50 of human EFNA5 protein is 0.8674-1.119ng/ml. Human EPHA3 protein His-Tag (CSB-MP007723HU) captured on COOH chip can bind human EFNA5 protein Fc-Tag (CSB-MP007464HU) with an affinity constant of 13.8nM as detected by LSPR Assay.
• Amino Acid Sequence:QDPGSKAVADRYAVYWNSSNPRFQRGDYHIDVCINDYLDVFCPHYEDSVPEDKTERYVLYMVNFDGYSACDHTSKGFKRWECNRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVHDRVFDVNDKVENSLEPADDTVHESAEPSRGEN
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.