The Integral membrane protein 2B (ITM2B) (Human) BioAssay™ ELISA Kit is for the in vitro quantitative measurement of Human Integral membrane protein 2B (ITM2B) concentrations in serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Detection Range:0.78-50ng/ml
Sensitivity:<0.4ng/ml
Test Principle:This kit is based on sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient ITM2B will produce a blue colored product, which then changes to yellow after adding the acidic stop solution. The intensity of the yellow color is proportional to the ITM2B amount bound on the plate. The Optical Density (OD) is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of ITM2B can be calculated.
Kit Components:Microtiter Strips, 1x96 wells. Pre-coated with anti-ITM2B antibody
Standard, 2xtubeWash Buffer (25X), 30mlSample/Standard Diluent Buffer, 20mlDetection Reagent A (100X), 120ulDetection Reagent B (100X), 120ulDiluent A, 10mlDiluent B, 10mlTMB Substrate, 10mlStop Solution, 10mlStorage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
