Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
Source:Recombinant protein corresponding to aa1-153 from Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE, fused to Flag-Tag at C-terminal, expressed in Mammalian cell.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, Mammalian cell
Grade：Purified
Purity：≥90% (SDS-PAGE)
Form：Supplied as a liquid in Tris, pH 8.0, 6% trehalose, 50% glycerol
