85-6101-03 Crossover Junction Endodeoxyribonuclease RuvC, Recombinant, E. coli, aa2-173, His-SUMO-Tag 100ug 584101
特徴
- Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.
- Source:Recombinant protein corresponding to aa2-173 from Escherichia coli Crossover junction endodeoxyribonuclease RuvC, fused to 6X His-SUMO-Tag at N-terminal, expressed in E.coli.
- Molecular Weight:~34.6kD
- Amino Acid Sequence:AIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSRLKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEYAARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQMSESRLNLARGRLR
- Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
- Size：100ug
- Source Antigen：Recombinant, E. coli
- Grade：Purified
- Purity：≥90% (SDS-PAGE)
- Form：Supplied as a liquid in Tris, pH 8.0, 6% trehalose, 50% glycerol
- Molecular Weight Non Ab：34.6
- Swiss Prot Number：P0A814
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