85-6088-65　5-keto-L-gluconate Epimerase, Recombinant, Thermotoga maritima, aa1-270, His-Tag, Myc-Tag 20ug　583441

特徴

• Catalyzes the reversible epimerization between 5-keto-L-gluconate (5-dehydro-L-gluconate) and D-tagaturonate, and thus probably functions in a myo-inositol degradation pathway together with IolG, IolM and IolN. Is not active on the enantiomer 5-keto-D-gluconate. Was also shown to be a nonphosphorylated sugar isomerase with broad substrate specificity in vitro. Is able to catalyze the reversible C3-epimerization of L-ribulose to L-xylulose, D-ribulose to D-xylulose, D-psicose to D-fructose, and D-tagatose to D-sorbose, with a substrate preference for ketopentoses rather than ketohexoses. Also catalyzes the aldose-ketose isomerization reaction from either D-erythrose or D-threose to D-erythrulose. Exhibits no activity for C4-epimerization of D-tagatose to D-fructose.
• Source:Recombinant protein corresponding to aa1-270 from Thermotoga maritima 5-keto-L-gluconate epimerase, fused to 10X His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E.coli.
• Molecular Weight:~37.9kD
• Amino Acid Sequence:MKLSLVISTSDAAFDALAFKGDLRKGMELAKRVGYQAVEIAVRDPSIVDWNEVKILSEELNLPICAIGTGQAYLADGLSLTHPNDEIRKKAIERVVKHTEVAGMFGALVIIGLVRGRREGRSYEETEELFIESMKRLLELTEHAKFVIEPLNRYETDFINTIDDALRILRKINSNRVGILADTFHMNIEEVNIPESLKRAGEKLYHFHVADSNRWAPGCGHFDFRSVFNTLKEIGYNRYVSVECLPLPGGMEEAAEIAFKTLKELIIKLT
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.