85-6086-89　Insulin, Bovine Pancreas 10mg　CAS No:11070-73-8　583350

特徴

• Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800D. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.
• Molecular Weight:5733.49
• Molecular Formula:C254H377N65O75S6
• Source:Bovine Pancreas
• Purity:≥98%
• Appearance:White to off-white solid
• Solubility:Water (11mg/ml) (1.92mM; ultrasonic and adjust pH to 2 with HCl)
• Amino Acid Sequence:Phe-Val-Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Gl u-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala. Gly-Ile-Val-Glu-Gln-Cys-Cys-Ala-Ser-Val-Cys- Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn (Disulfide bridge: Cys7-Cys7', Cys19-Cys20', Cys6')
• Biological Activity:27u/mg
• Endotoxin:≤10EU/mg
• Loss on Drying:≤10%
• Zn Content:As Reported
• Applications:Suitable for use in cell culture applications at 0.5ml/liter of medium.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -80°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -80°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.