85-6086-69　［取扱停止］Zika Virus Lysate, Strain PRVABC59 100ug　497260

特徴

• Zika virus is an enveloped single stranded RNA virus. It is a member of the Flavivirus genus of the family Flaviviridae which also includes global human pathogenic viruses Dengue virus (DENV), Yellow Fever virus, West Nile virus and Japanese encepahalitis virus. Zika is an emerging disease caused by Zika virus, which is transmitted through the bit of infected Aedes mosquitoes. The virus was first isolated in Central Africa, and has since spread to South Asia and recently to South America. Outbreaks were reported in Micronesia in 2007 and in Brazil in 2015 and has now been reported in 86 countries and territories.
• The Zika virus outbreak in Brazil gained world-wide attention, when reports suggested a link to an increasing number of microcephaly cases in the developing foetus and newborns. In April 2016, the Centers for Disease Control and Prevention in the USA, confirmed that the Zika virus can be transmitted to the fetus during pregnancy causing microcephaly (CDC). The Zika virus can also be transmitted to individuals through sexual contact, blood product transfusion and organ transplant.
• Healthy individuals infected with Zika virus experience a mild fever, rash, myalgia, arthralgia and headaches, with one in four infected individuals being asymptomatic. Due to similar symptoms Zika virus infected individuals can easily be misdiagnosed as a dengue infection and vice-versa. Currently, there is no vaccine or specific treatment available for Zika virus.
• There is currently a major worldwide effort underway to develop effective diagnostic assays that can both detect Zika virus infection, and distinguish infection from other flaviviruses, especially Dengue virus infection. This Zika virus cell lysate has been prepared to offer a broad range of Zika virus antigens that may be relevant for the detection of Zika virus specific antibodies.
• Source: Zika Virus Lysate, strain PRVABC59 strain, propagated in the LLC-mk2 or Vero cell line
• Applications: Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Inactivation:Purified virions were lysed in 0.6M KCl containing 0.5% Triton X-100, and then subjected to heat inactivation.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.