85-6086-67　［取扱停止］Yellow Fever Virus Lysate, 17D Strain 100ug　497258

特徴

• Yellow fever is an acute hemorrhagic disease caused by the yellow fever virus (YFV), which is a member of the Flaviviridae family of viruses. Clinical symptoms of the disease include fever, muscle pain, nausea and vomiting. In a small percentage of patients, the liver and kidneys are affected leading to jaundice, and in some cases death.
• Yellow fever virus is endemic in tropical areas of Africa and Central/South America where the vector is widespread. In the sylvatic cycle, the virus is transmitted to non-human primates via mosquitoes of the Hemagogus and Sabethes genera, with occasional outbreaks of Yellow Fever in individuals working in forest areas. Whereas the Aedes aegypti mosquito is responsible for the transmission of Yellow Fever virus to humans in urban areas.
• In the late 1930’s a safe and effective attenuated vaccine was developed against the YFV, which confers long-term immunity (Norrby, E). The virulent wild-type Asibi strain of YFV was used to develop the YFV 17D vaccine, which is now commonly used to successfully to immunize individuals at risk of YFV infection (WHO).
• Diagnosis of yellow fever is complicated by the fact that early symptoms of the infection can be confused with other hemorrhagic diseases including Dengue. Differential diagnosis is therefore an important consideration in areas where other flaviviruses such as Dengue and Zika co-circulate.
• Source: Yellow Fever Virus Lysate, strain Yellow Fever Virus (17D strain), expressed in Vero cell line.
• Applications:Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Inactivation:Purified virions were lysed in 0.6M KCl containing 0.5% Triton X-100, and then subjected to heat inactivation.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.