Wesselsbron disease (WSL) is an arthropod-borne flavivirus infection, primarily of domestic livestock (sheep, bovine, and goats) but also in humans, where it causes a non-fatal influenza-like illness. No fatal infections have yet been reported in humans. The Wesselsbron virus (WSLV) has not been well characterized, but it has the properties that are typical of a hemagglutinating flavivirus and is widespread in Africa.
Subclinical infections are believed to be common among people in sub-Saharan Africa with seroprevalence rates of 1% to 35%. However, cross-reactions between flaviviruses may cause false positive reactions in some cases. Serological tests available include hemagglutination inhibition (HI), complement fixation, virus neutralization and ELISA, and although there is a high degree of cross-reactivity with other flaviviruses in HI tests, Wesselsbron titers are generally higher than other flavivirus titers. In 2010-2011, two cases of Wesselsbron disease were confirmed in livestock farmers during an outbreak of Rift Valley fever.
WSLV is one of a number of lesser known mosquito transmitted flaviviruses which are believed to have the potential to emerge (Smith, 2017). This WSLV NS1 protein has been manufactured in response to the unmet need for a highly purified, concentrated protein for use in further vaccine development and serological based diagnostic assays. The detection of the NS1 protein offers much higher fidelity across multiple flaviviruses where cross-reactivity can otherwise pose an issue.
Recombinant protein corresponding to aa750-1123 from Wesselsbron Virus NS1 Protein, fused to 6X His-Tag at C-terminal, expressed in HEK293 cells.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
