85-6086-39　［取扱停止］Respiratory Syncytial Virus B Lysate 100ug　497202

特徴

• Respiratory syncytial virus (RSV, also known as human orthopneumovirus) is a negative-sense, single-stranded RNA virus belonging to the Pneumoviridae family. RSV is a medium-sized virus (120-200nm) and includes a viral envelope. The RSV genome is approximately 15kb in length and encodes 10 genes and 11 proteins. From 3-5’, the genome encodes the non-structural proteins 1 and 2 (NS1, NS2), Nucleoprotein (N), Phosphoprotein (P), Matrix protein (M), Small hydrophobic protein (SH), Glycoprotein (G), Fusion protein (F), Matrix protein 2 (M2), and Large protein (L) (Borchers et al. 2013). There is only one RSV serotype, which includes the major antigenic subgroups A and B (Gilca et al., 2006). In temperate climates, RSV transmission shows a distinct seasonality with typical onset in late autumn to early winter, and a peak between mid-December and early February. In the USA, 60% of infants are infected during their first HRSV season It is generally believed that most children will have experienced at least one RSV infection by the age of 2 years (Borchers et al. 2013). Of those infected, 2–3% will develop bronchiolitis and require hospitalisation. The resulting natural immunity is protective but wanes over time, such that people can be infected multiple times. Possible co-infection of respiratory syncytial virus and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been reported (Burstein et al., 2020).
• Source: Respiratory Syncytial Virus B Lysate, expressed in Vero cells.
• Applications: Suitable for use in Western Blot and Dot Blot. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Inactivation:Viral inactivation is verified for every lot of lysate by the absence of viral growth in validated tissue culture-based infectivity assays.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.