85-6086-35　［取扱停止］Plasmodium Falciparum HRP-II Protein, Recombinant 100ug　497198

特徴

• Plasmodium falciparum is a unicellular protozoan parasite of humans, and causes the most dangerous form of malaria, with the highest rate of complications and mortality in humans. It is transmitted through the bite of female Anopheles mosquitos and is responsible for ~50% of all malaria cases worldwide. As a result, P. falciparum is regarded as the deadliest parasite in humans, causing 435,000 deaths in 2017 alone (WHO).
• Histidine-rich protein II (HRPII) is exclusively produced by the falciparum species of Plasmodium and is essential during the intra-erythrocytic stages of its parasitic life cycle. HRPII is so-called because 37% of its amino acid sequence comprises histidine residues (Pal et al., 2016). HRPII is exported by the parasite into the erythrocyte cytosol, where it is then released into the bloodstream upon cell lysis. Here it is thought to circulate act as a virulence factor by compromising the endothelial barrier integrity of the CNS and cause cerebral malaria (idib). Since HRPII is expressed only by the falciparum species and accumulates to high levels in the bloodstream, it can be used as a marker to distinguish between a P. falciparum malaria infection and infection with one of the other four non-falciparum species (Pal et al., 2017).
• Source:Recombinant protein corresponding to Plasmodium Falciparum HRP-II Protein, expressed in E. coli.
• Molecular Weight:~60kD
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.