Porcine parvovirus (PPV) causes reproductive failure in pregnant sows. The infection occurs without clinical symptoms in adults; however, the virus can cross the placental barrier during the infection and cause the death of the fetuses, stillbirths and return to estrus (Streck et al., 2020). PPV is also able to increase the effects of porcine circovirus type 2 infection in the clinical course of postweaning multisystemic wasting syndrome (PMWS), another significant disease in global swine production (Ouyang et al., 2019).
PPV2 (proposed name Ungulate tetraparvovirus 3), a member of the Tetraparvovirus genus, was discovered in 2001 during a survey for hepatitis E virus (HEV) in swine sera collected in Myanmar (Hijikata et al., 2001; Cságola et al., 2016). PPV is a small, non-enveloped, single-stranded, negative-sense DNA virus. Capsids of PPV are assembled from three viral proteins (VP1, VP2, and VP3). The capsid is a spherical shell (~28nm in diameter) consisting of 60 identical copies of viral proteins arranged in an icosahedral symmetry. These identical copies (subunits) comprise about 90% of VP2 and 10% of VP1 molecules. The major structural protein, VP2 is the main target for neutralizing antibodies in PPV. Because VP2 is the main structural protein of PPV and constitutes most of the viral capsid, VP2 produced in vitro can self assemble into virus-like particles. For example, when VP2 is expressed using the baculovirus expression vector system, it assembles into virus-like particles (VLPs) similar in size and morphology to native virions and seen as a 64kD band by Western-blot analysis. PPV VP2 VLPs have been shown to induce antibodies against PPV in immunized pigs and rabbits. PPV-cell or tissue-tropism determinants, host-range determinants, and determinants that confer hemagglutination properties have all been shown to be located in the capsid proteins. PPV VP2 VLPs also exhibited positive immunoreactivity for PPV in a commercial ELISA (Mészáros et al., 2017; Zhou et al., 2010).
Recombinant PPV-His-VP2 expression confirmed in infected cells by Western with α-His specific antibodies. Tested in ELISA with α-PPV specific antiserum.
Source:Recombinant protein corresponding to 585aa from Parvovirus 2 Capsid Protein 1, fused to 6X His-Tag at N-terminal, expressed in insect cells.
Molecular Weight:~65.2kD
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
