85-6086-31　［取扱停止］Pan Plasmodium Aldolase, Recombinant 100ug　497194

特徴

• P. falciparum Aldolase is a 41kD enzyme that binds to members of the thrombospondin-related anonymous protein (TRAP) family, including Actin and Adhesin. By doing so, Aldolase couples the actomyosin motor and cell surface adhesins involved in motility and host cell (Diaz et al., 2016) Aldolase also helps convert glucose into energy, making it critical for the intraerythrocytic merozoite life stage. Higher vertebrates usually have multiple tissue-specific aldolase isoenzymes. However, P. falciparum and P. vivax have only one aldolase isoenzyme, which is highly conserved at the amino acid sequence and structural level. Therefore, P. falciparum Aldolase is used as one of the markers for diagnosis of malaria infection.
• Plasmodium falciparum is a unicellular protozoan parasite of humans, and causes the most dangerous form of malaria, with the highest rate of complications and mortality in humans. It is transmitted through the bite of female Anopheles mosquitos and is responsible for ~50% of all malaria cases worldwide. As a result, P. falciparum is regarded as the deadliest parasite in humans, causing 435,000 deaths in 2017 alone (WHO).
• Plasmodium falciparum Aldolase is composed of the full-length amino acid sequence of the P. falciparum Aldolase protein, which is cloned into the bacteria expression vector. The soluble recombinant protein is purified from E. coli with affinity chromatography to ≥95% purity.
• Molecular Weight:~66kD
• Applications:Suitable for use in Immunoassays. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.