Nipah virus (NiV) is an emerging and highly pathogenic zoonotic paramyxovirus (genus Henipavirus) that is transmitted by its natural reservoir, Pteropid bats. NiV initially emerged in 1998 in Malaysia, and is now distributed in Asia, Africa, and the South Pacific Ocean. It causes deadly respiratory and encephalic diseases in humans. Because its mortality rate can exceed 70% in humans, its potential for human-to-human transmission and the absence of vaccine or specific antiviral treatment, it is classified as a biosafety level 4 (BSL-4) pathogen (Yabukarski et al., 2014).
The NiV genome consists of a negative-sense, single-stranded RNA of approximately 18.2kb, encoding six structural proteins, nucleoprotein (N), phosphoprotein (P), matrix protein (M), fusion protein (F), attachment glycoprotein (G), and the large protein or RNA polymerase protein (L). In addition, the P gene encodes three non-structural proteins by RNA editing (V and W proteins) or an alternative open reading frame (C protein) (Wang et al. 2001).
NiV genomic RNA is condensed by a homopolymer of nucleoprotein (N), forming long helical nucleocapsids (NCs). The nucleoprotein encapsidates the genome protecting it from nucleases. Ribonucleoprotein complexes (RNP) are then used as a template for RNA synthesis by the viral RNA-dependent RNA polymerase (Yabukarski et al., 2014). Within the RNP, N is responsible for viral genome wrapping and facilitates viral replication and transcription (Lee et al., 2012).
Source:Recombinant protein corresponding to aa1-532 from Nipah Virus Nucleoprotein, fused to His-Tag at C-terminal, expressed in HEK293 cells.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293 cells
Grade：Purified
Purity：≥90%
Form：Supplied as a liquid in 50mM HEPES, pH 8, 150mM sodium chloride, 4M urea.
