85-6086-22　［取扱停止］Metapneumovirus 18 Type B2 Lysate, Strain IA18-2003 (hMPV) 100ug　497185

特徴

• Human metapnuemoviruses (hMPV) are negative-sense, single-stranded RNA viruses of the family Pneumoviridae. hMPV was first isolated in 2001 in the Netherlands using RAP-PCR for identification of unknown viruses growing in cultured cells. hMPV infection is the second most common cause of lower respiratory tract infections in young children after respiratory syncytial virus (RSV). Most infants who are hospitalised with hMPV infection are between 6–12 months of age and the clinical features and severity of HMPV are similar to those of RSV. The genomic organisation of hMPV is similar to RSV, however, hMPV lacks the non-structural genes, NS1 and NS2, and the hMPV antisense RNA genome contains eight open reading frames in slightly different gene order than RSV (viz. 3’-N-P-M-F-M2-SH-G-L-5’) (van den Hoogen et al., 2002). The genome is about 13kb in size and is composed of eight genes encoding nine proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), fusion protein (F), matrix-2 proteins (M2-1 and M2-2), small hydrophobic (SH) protein, glycoprotein (G), and large (L) polymerase protein. hMPV is genetically similar to the avian metapneumoviruses A, B and in particular type C. Phylogenetic analysis of hMPV shows there are two main genetic lineages, termed A and B, which contain within them the subgroups A1/A2 and B1/B2, respectively (Shafagati et al., 2018).
• Source: Metapneumovirus 18 Type B2 Lysate, strain IA18-2003, expressed in LLC-mk2 cell line.
• Applications: Suitable for use in Western Blot and Dot Blot. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.