Measles virus (MV) is the type species of the Morbillivirus genus, which is one of the seven genera of the family Paramyxoviridae. It is a highly contagious, re-emerging, major human pathogen. It is a globally widespread disease that affects children but can also cause disease in unvaccinated adults. Humans are the only known host of measles virus and infection is spread from person-to-person via respiratory aerosol droplets, nasal secretions or through direct contact with infected individuals.
MVs are enveloped and contain single-stranded, negative-sense RNA genomes of 9-19kbp. The viral RNA is bound by thousands of copies of the viral nucleoprotein, forming the helical nucleocapsid (NC). The MV single-stranded RNA genome is enclosed by nucleocapsid proteins into helical ribonucleoprotein (RNP) complexes comprising of nucleo- (N), phospho- (P), and large (L) proteins. Encapsidated RNAs act as templates for transcription and replication by the viral RNA-dependent RNA-polymerases (RdRPs) complexes (Gutsche et al., 2015). Unencapsidated viral genomic RNA is transcriptionally inactive and not infectious (Duprex et al., 2002). N protein comprises two distinct parts: Folded Ncore and Ntail, an intrinsically disordered domain, containing a linear motif with α-helical propensity (residues 484–502) (Guseva et al., 2019).
MeV has an RNA-dependent RNA polymerase composed of two viral polypeptides, a large protein, L, and a phosphoprotein, P, for transcription and replication of their genome. The L protein confers the RNA polymerase activity on the complex while the P protein acts as a transcription factor. Replication requires a viral RNA-dependent RNA polymerase (RdRP) consisting of the large (L) polymerase protein complexed with the homo-tetrameric phosphoprotein (P) (Du Pont et al., 2019).
Source:Recombinant protein corresponding to aa1-523 from Measles Virus Nucleoprotein, expressed in HEK293 cells.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
