85-6086-15　［取扱停止］Influenza B Nucleoprotein, Recombinant [B/Beijing/184/93] 100ug　497178

特徴

• Of the three types of influenza virus (A, B and C) A is the best characterized and the most serious threat to public health. One of the three core Influenza polypeptides is an RNA-dependent RNA polymerase and a single-strand RNA (ssRNA) binding protein, often referred to as a nucleoprotein (NP). NP encapsidates the virus genome to form a ribonucleoprotein (RNP) particle for the purposes of transcription and packaging. NP is not just the structural component of the virus transcription machinery but performs multiple essential functions throughout the virus life cycle (Portela & Digard, 2002). Influenza A virus RNA segment 5 encodes NP is a polypeptide of 498aa in length; in influenza B and C viruses, the length of the homologous NP polypeptide is 560aa and 565aa residues, respectively (Londo et al., 1983; Nakada et al., 1984). Alignment of the predicted amino acid sequences of the NP genes of the three influenza virus types reveals significant similarity among the three proteins, with the type A and B NPs showing the highest degree of conservation. Phylogenetic analysis of virus strains isolated from different hosts reveals that the NP gene is also well conserved, with a maximum amino acid difference of less than 11% (Shu et al., 1993). The primary sequence of NP is modified by phosphorylation (Privalsky & Penhoet, 1978) and the phosphopeptide pattern of NP is strain-specific, dependent on the host cell used for propagating the virus and changes throughout virus replication (Kistner et al., 1985, 1989). Seasonal influenza viruses display high variability and because of the threat of influenza viruses with pandemic potential, there is great interest in the development of vaccines that induce broadly protective immunity, such as those based on conserved proteins, including nucleoprotein. NP has also been shown to induce cross-reactive antibody and T cell responses (Altenburg et al., 2017).
• Source:Recombinant protein corresponding to Influenza B Nucleoprotein, strain Influenza B/Beijing/184/93, expressed in E. coli.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.