85-6086-08　［取扱停止］Influenza A H1N1, Strain Influenza A/New Cal/20/99 [A/New Cal/20/99] 100ug　497171

特徴

• The influenza A viruses are negative-sense, single-stranded, segmented RNA viruses of the genus Alphainfluenzavirus, family Orthomyxoviridae. There are several subtypes, named according to the type of Hemagglutinin (H1-18) and Neuraminidase (N1-11) (Centers for Disease Control and Prevention, 2017). Humans are generally infected by influenza viruses of the subtypes H1, H2 or H3, and N1 or N2. Minor point mutations causing small changes (known as antigenic drift) occur relatively often and enable the virus to evade immune recognition, resulting in repeated influenza outbreaks during interpandemic years. Significant mutations in the HA antigen (antigenic shift) are caused by the reassortment of genetic material from different co-infecting A subtypes. Each virus subtype has also mutated into various strains with differing pathogenic profiles; some are pathogenic to one species but not others, and some are pathogenic to multiple species.
• The influenza A virus subtype H1N1 is most well-known for causing the 1918-1919 Spanish Flu pandemic and the 2009 Swine Flu pandemic, resulting in approximately 50 million and 300,000 deaths, respectively (Centers for Disease Control and Prevention, 2012). Influenza A/New Caledonia/20/99 (H1N1) was originally isolated on June 9, 1999 from a human nasal swab in New Caledonia in the South Pacific and has since been used as the influenza A (H1N1) component of trivalent vaccines worldwide from 2000-2007 (WHO).
• Source: Influenza A H1N1, strain Influenza A/New Cal/20/99, expressed in MDCK cell line.
• Applications: Suitable for use in Western Blot and Dot Blot. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Inactivation:Heat inactivated. Viral inactivation is verified for every lot of lysate by the absence of viral growth in validated tissue culture-based infectivity assays.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.