85-6086-05　［取扱停止］Immunodeficiency Virus Tat Protein, Recombinant, His-Tag [HIV-1/Clade C (TV019)] 50ug　497168

特徴

• Immediately after HIV infects a cell, the virion RNA is copied into DNA and the proviral genome is transported to the nucleus and integrated into the host cell genome. Once integrated into the host chromosome, the HIV becomes subject to regulation by cellular transcription factors, as well as its own regulatory proteins. HIV transcription is controlled primarily by the trans-activator protein (Tat). Tat is one of two essential viral regulatory factors (Tat and Rev) for gene expression. Two forms are known, Tat-1 exon (minor form, 72aa) and Tat-2 exon (major form, 86aa) and low levels of both proteins are found in persistently infected cells. Tat has been localized primarily in the nucleolus/nucleus by immunofluorescence. It acts by binding to the TAR RNA element and activating transcription initiation and elongation from the LTR promoter, preventing the 5′ LTR AATAAA polyadenylation signal from causing premature termination of transcription and polyadenylation. It is the first eukaryotic transcription factor known to interact with RNA rather than DNA and may have similarities with prokaryotic anti-termination factors. Tat can be secreted by infected cells and extracellular Tat can be taken up by cells in culture through interactions with heparin sulfate proteoglycans displayed on their surface. Subsequently, Tat reaches their nucleus and can activate a variety of cellular genes including cytokines and cytokine coreceptors.
• Recombinant protein corresponding to full-length HIV-1 Tat Clade B, expressed in E. coli
• Applications:Suitable for use in Western Blot. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.