Hantaan orthohantavirus virus is a member of the Hantavirus genus within the Bunyaviridae family of viruses and is a causative agent of a potentially fatal hemorrhagic fever and renal syndrome. Hantavirus genomes have three segments of single stranded negative-sense RNA, named small (S), medium (M) and large (L). The L segment encodes the viral polymerase, the M segment encodes the precursor (GPC) for two viral surface glycoproteins (Gn and Gc), and the S segment encodes the nucleocapsid (N) protein. The hantavirus N plays a pivotal role in viral replication, transcription and assembly (Muyangwa, M).
The Hantaan virus nucleocapsid protein has a molecular weight of ~50kD and forms a ribonucleoprotein complex which encapsidates genomic RNAs. The Nucleocapsid localises at the perinuclear region of infected or N-expressing cells, forming inclusion bodies and filamentous structures (Yoon, et al., 2015). The nucleoproteins N-terminal contains two coiled-coil motifs that facilitate trimerization to produce putative virus particle assembly intermediates (Alfadhli, et al., 2001). Studies have also demonstrated that in hantavirus-infected cells, N interacts with various cellular host proteins, including Daxx, Ubc and SUMO-1 (Li, et al., 2002; Kaukinen, et al., 2003).
Nucleoproteins are relatively conserved among hantaviruses and are highly immunogenic, having been shown to induce efficient protective immunity in animal models (Carvalho, et al. 2002). The immune responses elicited by NPs are thought to be mainly cellular, as N-specific antibodies have not been shown to be neutralizing. However, specific anti-N antibodies have been shown to induce partial protection in passive immunization experiments with monoclonal antibodies, indicating that N-specific antibodies can contribute to inactivation of hantaviruses through other mechanisms (Carvalho, et al. 2002).
Source:Recombinant protein corresponding to aa1-125 from Hantaan Virus Nucleoprotein, fused to His-Tag at N-terminal, expressed in E. coli.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Form：Supplied as a liquid in PBS, 0.05% sodium azide.
