85-6085-84　［取扱停止］Feline Calcivirus Antigen, Strain Bolin 100ug　497146

特徴

• Feline calicivirus (FCV), a small, unenveloped RNA virus, is one of the most common viral pathogens of cats. It has a non-enveloped, 35-40 nm, positive-sense, single-stranded RNA genome. Open reading frames 1, 2, and 3 encode a helicase, a protease, and polymerase; the single capsid protein; and an RNA-associated structural protein, respectively. It is enclosed by multiple copies of the major capsid protein, which is the target of protective immune responses. Hypervariable regions in this gene confer antigenic variability that allows viral persistence in the face of a developing immune response. In persistently infected cats, the gene for the major structural protein of the viral capsid has been shown to evolve through immune-mediated positive selection, which allows the virus to escape detection by the immune system (Coyne et al., 2006).
• FCV is endemic in most catteries, shelters, and large multiple-cat households (Radford et al., 2007). Infection generally occurs through direct contact with secretions from acutely infected and carrier cats. FCV also survives much better in the environment (~1 month) than other viruses e.g. feline herpesvirus 1 (FHV-1), increasing the risk of transmission. Different strains of FCV can vary in virulence resulting in great variability of clinical signs. FCV strains comprise one serotype and predominantly and only two genotypes worldwide, although there is considerable variation between strains, which has an impact on vaccination (reviewed in Green at al., 2000). Most strains used in vaccines protect against the majority of isolates, but not equally well against all, including some of the more recently emerged virulent systemic feline calicivirus (VS-FCV).
• Source: Feline Calcivirus Antigen, strain Bolin, expressed in FC 3TG cells.
• Applications: Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Inactivation:UV and detergent inactivated.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.