85-6085-83　［取扱停止］Epidemic Diarrhea Virus Nucleoprotein, Recombinant, Porcine, His-Tag 100ug　497145

特徴

• Porcine epidemic diarrhea virus (PEDV), an enveloped, single-stranded and positive-sense RNA virus, is the causative agent of porcine epidemic diarrhea (PED). PEDV belongs to the genus of Alphacoronavirus, the family of Coronaviridae and the subfamily of Coronavirinae. It was originally reported in Belgium and the United Kingdom and infection causes 80 to 100% fatality rate in suckling piglets (Jung et al., 2020).
• PEDV has an approximately 28kb long genome and consists of seven open reading frames (ORF), encoding non-structural or structural proteins. PEDV encodes several structural proteins, including the spike (S), envelope (E), membrane (M), and nucleoprotein (N). The N protein, which is the most abundant protein in the virus particle, provides the structural basis for the helical nucleocapsid surrounding the virus genome. The nucleoprotein also assists the correct folding, packaging and encapsidation of viral RNA. It can also regulate the host cell cycle to benefit virus infection. During the infection, PEDV nucleoprotein protein (PEDV-N) is mainly localized in the cytoplasm, but can also be found to a lesser extent in the nucleus. PEDV-N also antagonizes interferon-beta production by sequestering the interaction between IRF3 and TBK1 (Huan et al., 2016).
• Recombinant PEDV-NP-His expression confirmed in infected cells by Western with α-His and α-PEDV-NP specific antibodies. Tested in ELISA with α-PEDV-NP specific antibodies.
• Source:Recombinant protein corresponding to 447aa from porcine Epidemic Diarrhea Virus Nucleoprotein, strain USA/Minnesote84/2013, fused to 6X His-Tag at N-terminal, expressed in insect cells.
• Molecular Weight:~49.8kD
• Applications:Suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.