Dengue virus is a member of the flavivirus family, which includes Zika virus, West Nile virus and Japanese Encephalitis virus. Many of the proteins expressed by these viruses are very similar, and serological testing for these viruses can be complicated by problems of cross-reactivity. In this context, the use of highly purified antigens, that are glycosylated and folded as native proteins can be highly important in the development of accurate immunoassays.
Dengue infection is caused by any one of four related Dengue virus serotypes DENV1-4, which are transmitted by mosquitoes and co-circulate in endemic areas. With more than one-third of the world’s population living in areas at risk of transmission, dengue infection is a leading cause of illness and death in the tropics and subtropics (WHO for Guidelines for Diagnosis, Treatment, Prevention & Control, 2009). As many as 100 million people are infected yearly. There are no vaccines available to prevent dengue virus infection. Therefore, early diagnosis of dengue and dengue serotypes is a vital step in patient management.
Dengue virus envelope protein is a major structural glycosylated protein that forms homodimers on the virion surface. Each envelope protein is organised into three domains, the central domain DI, the dimerization domain DII and an immunoglobulin-like domain DIII. Envelope protein DIII is reported to be a receptor-recognition and binding domain, and an important target for the human immune response (Matsui, K et al). The DIII domain is highly immunogenic and elicits the production of neutralizing antibodies against the wild-type virus.
Recombinant protein corresponding to Dengue virus serotype 2 DIII envelope protein, produced in E. coli.
Applications:Suitable for use in ELISA, Western Blot and Lateral Flow. Other applications not tested.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in 20mM carbonate buffer, pH 9.6.
