85-6085-50　Canine Parvovirus 2 VP2 Capsid Protein, Strain Type 2b, Recombinant 100ug　497106

特徴

• Canine parvovirus (CPV) Canine parvovirus type 2 (CPV‑2) causes severe and highly contagious disease in dogs. CPV-2 is a non-enveloped single-stranded DNA virus in the Parvoviridae family, 20-26nm in diameter with an icosahedral symmetry. It is a relatively new virus that was first recognized in 1978 and then spread worldwide in only 1-2 years (Carmichael, 2005). CPV is highly contagious and is transmitted by the faecal-oral route through contact with feces from infected canine or contaminated surfaces. It is the most important enteric virus infecting canids.
• CPV‑2 has a non‑enveloped spherical capsid assembled from three proteins (VP1, VP2 and VP3). Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of a combination of VP1 (5‑6 copies) and VP2 (54‑55 copies). VP1 and VP2 are products of the same gene that are generated by alternative splicing of viral mRNA but VP1 has an additional 143aa in its N‑terminal compared to VP2. Empty capsids can be assembled from VP2 alone. VP3 is present only in full virions and is formed as the result of proteolytic cleavage of 19aa from the N‑terminal of VP2. The capsid encapsulates the genomic ssDNA and capsid proteins are responsible for the attachment to a host cell receptor. Phylogenetic analysis showed that all CPVs derived from a single common ancestor, and CPV-2 likely arose when it acquired mutations that allowed binding to the canine transferrin receptor (TfR) type-1. TfR plays a key role in the susceptibility of cells to infection by parvoviruses (reviewed in Miranda & Thompson, 2016). This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminal might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal may allow nuclear import of capsids.
• Source:Recombinant protein corresponding to 584aa of Type 2b strain from Canine Parvovirus 2 VP2 Capsid Protein, expressed in insect cells.
• Molecular Weight:~65kD
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.