Canine distemper is a disease in Canidae (canine, fox, wolf, raccoon canine), Mustelidae (ferret, mink, skunk, wolverine, marten, badger, otter), most Procyonidae (raccoon, coatimundi), some Viveridae (binturong, palm civet), Ailuridae (red panda), Ursidae (bear), Elephantidae (Asian elephant), primates (Japanese monkey), and large Felidae. Domestic canine (including feral populations) are considered to be the reservoir species in most, if not all, locations.
The disease agent is Canine distemper virus (CDV), a member of Morbillivirus genus in the family Paramyxoviridae, is a significant viral disease of both captive and free-ranging carnivores globally and closely related to the viruses of measles and rinderpest. The fragile, enveloped, single-strand RNA virus is relatively unstable outside the host (Loots et al., 2017). The main route of infection is via aerosol droplet secretions from infected animals and, once infected, canine may shed virus for several months. The virus initially replicates in the lymphatic tissue of the respiratory tract. A cell-associated viremia results in infection of all lymphatic tissues, which is followed by infection of respiratory, GI, and urogenital epithelium, as well as the CNS and optic nerves. Disease follows virus replication in these tissues. The degree of viremia and extent of viral spread to various tissues is moderated by the level of specific humoral immunity in the host during the viremic period. Overall, the mortality rate is around 50%, with death occurring 2 weeks to 3 months post-infection. With the potential increasing virulence of emerging strains and the wide host range of canine distemper virus, widespread vaccination of domestic canine is considered essential (Martinez-Gutierrez & Ruiz-Saenz, 2016).
Source: Canine Distemper Virus, strain Lederle, from SL-29 cells.
Applications: Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:UV and detergent inactivated.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
