Canine coronavirus (CCoV) belongs to the genus Alphacoronavirus and forms a unique species, Alphacoronavirus 1, along with feline coronaviruses (FCoVs), transmissible gastroenteritis virus of swine (TGEV), and its derivative, porcine respiratory coronavirus (PRCoV) (Carstens, 2010; Adams & Carstens, 2012). CCoV causes a highly contagious disease of dogs that primarily attacks the intestinal tract. It was discovered in 1971 in Germany during an outbreak in guard canines (Pratelli, 2006). More recently, strains of this enteric canine coronavirus have been identified with different properties, including pantropic strains of the enteric virus (Decaro et al., 2013).
CCoV invades and replicates in the villi of the small intestine and infection is difficult to identify; symptoms often resemble those of other viruses such as canine parvovirus and canine distemper virus. It causes diarrhea, lethargy and decreased appetite. The disease is also highly contagious with an incubation period of 1-3 days and is spread through the feces of infected canines, who usually shed the virus for six to nine days, but sometimes for six months following infection.
There are two strains of canine coronavirus (the enteric group 1 strain and the respiratory group 2 strain) which produce different symptoms. Canine respiratory coronavirus (CRCoV, Group II) is similar to strain OC43 of bovine and human coronaviruses and was first isolated in 2003 from canine lung samples in the UK (Erles et al., 2003). CRCoV is a part of the kennel cough complex and is spread in kennel-like situations where canine have high contact with one another or infected surfaces. Canine that catch it have mild symptoms such as cough, sneezing, and nasal discharge. Unlike canine respiratory coronavirus, a vaccine exists against canine enteric coronavirus (CCV), but it does not cross-protect against the respiratory strain.
Source: Canine Coronavirus, strain 1-71, expressed in A-72 cells.
Applications: Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Inactivation:UV and detergent inactivated.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Source Antigen：A-72 cells
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 50Mm glycine, pH 9.0, 140mm sodium chloride, <0.1% CHAPS, <0.01% SDS.
