The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sacrolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Alpha-dystroglycan is an extracellular peripheral glycoprotein that acts as a receptor for extracellular matrix proteins containing laminin-G domains.
Lassa fever virus (LAFV) exhibits broad tissue tropism and its major cellular receptor is extracellular matrix receptor dystroglycan (DG). DG is a ubiquitously expressed and highly conserved receptor for extracellular matrix (ECM) proteins, where it provides a molecular link between the ECM and the actin-based cytoskeleton (Cao et al., 1998). DG is cleaved into the extracellular alpha-DG (alpha-DG) and membrane-anchored β-DG (Barresi and Campbell, 2006). In mammals, alpha-DG is also subject to complex O-glycosylation, which is essential for its function as a receptor for ECM proteins and arenaviruses (Yoshida-Moriguchi and Campbell, 2015; Kunz et al., 2005; Rojek et al., 2007). Binding of LASV depends on DG’s tissue-specific posttranslational modification with the unusual O-linked polysaccharide matriglycan. However, functional glycosylation of DG does not always correlate with viral tropism observed in vivo, suggesting the existence of alternative receptors. The broadly expressed phosphatidylserine (PS) receptors Axl and Tyro3 have also been identified as alternative LASV receptor candidates (Fedeli et al., 2018).
Alpha-dystroglycan also acts as a receptor for lymphocytic choriomeningitis virus glycoprotein and class C new-world arenaviruses (Imperiali et al., 2005, Kunz, 2009; Rojek et al., 2007). It is also the Schwann cell receptor for Mycobacterium leprae, the causative organism of leprosy, but only in the presence of the G-domain of LAMA2 (Rambukkana et al., 2008).
Source:Recombinant protein corresponding to aa28-651 from DAG at the C-terminal transmembrane domain and the cytoplasmic tail replaced by 15 aa Glycine-Serine linker and human IgG1 Fc-tag, expressed in HEK293 cells.
Applications:Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, HEK293 cells
Grade：Purified
Purity：Purified by Immobilized metal affinity chromatography.
