African swine fever virus (ASFV) is a large DNA virus and the only member of family Asfarviridae (Dixon et al., 2013). It infects domestic pigs, wild boars, warthogs, and bush pigs, as well as soft ticks (Ornithodoros erraticus), which likely act as a vector. African swine fever (ASF) is characterized by high morbidity and mortality. It is spreading across Europe and in 2018 was introduced into China, leading to high mortality in domestic pigs (Bao et al., 2019). There are no commercial vaccines available for this disease and so control measures are heavily dependent on detection of nucleic acid, antigen, and antibodies.
The genome of ASFV ranges in length from ~170-193 kbp depending on the isolate and contains between 150 and 167 open reading frames (ORFs), which encode 150-200 proteins, with ~50 of them structural, including p30, pp62 and p72 (Jia et al., 2017). For the purpose of serologic detection, p72 antigen is one of the most immunogenic ASFV proteins (Kollnberger et al., 2002), being the major capsid component of viral icosahedrons. It is very important in forming the viral capsid in late stage expression of virus infection (Neilan et al., 2004). The middle portion of the p72 protein contains most epitopes tested by monoclonal antibodies, whilst the C-terminal peptide of p72 is generally used for genotyping ASFV isolates by sequencing (Jia et al., 2017; Heimerman et al., 2018).
Recombinant protein corresponding to aa40-315 from African Swine Fever Virus P72 Protein, fused to His-Tag at C-terminal, expressed in E.coli.
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Molecular Weight:~33kD
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：≥95% (10% SDS-PAGE, Coomassie stain)
Form：Supplied as a liquid in 25mM Tris Base, 10mM potassium carbonate.
