85-6085-34　［取扱停止］Rapid Ligation Kit, BioGenomics™ 100Rxns　583343

特徴

• Our yT4 DNA ligase catalyzes the joining of two strands of DNA between the 5′ – phosphate and the 3′ – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. The enzyme also repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. The Rapid Ligation Kit is designed for efficient ligation of DNA inserts into vectors in just 5 minutes.
• Features:Either sticky- or blunt-end, ligation can be achieved within 5 minutesThe reaction mixture can be used directly for bacterial transformation
• Applications:Join double-strand DNA with cohesive or blunt termini
• Join oligonucleotide linkers to DNA sequence with the blunt end
• Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids.
• Kit Components:yT4 DNA ligase, concentration 3U/ul, 1x100ul10X Ligation Buffer A, 200ul10X Ligation Buffer B, 200ul
• yT4 DNA ligase Storage Buffer:20mM Tris-HCl (pH 7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA and50% glycerol.
• 10X Ligase Buffer A:0.4M Tris-HCl, 0.1mM MgCl2, 0.1M DTT and 5mM ATP (pH 5.0 at25°C)
• 10X Ligase Buffer B:A buffer contains an enhancer which dramatically increases ligationefficiency for blunt end DNA
• Unit Definition:One unit of enzyme catalyzes the conversion of 1 nanomole of [32PPi]into Norit-adsorable form in 20 min at 37°C (Weiss unit).
• Storage and Stability:-20°C