85-6085-29　［取扱停止］ECOS™ 9-5 Competent Cells [JM109] Efficiency:>5x10e7 cfu/ug 10x100ul　583338

特徴

• JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
• This product can become a powerful cloning tool when it is used in combination with our rapid ligation kit. The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours.
• Features:Rapid growing strain, carries F’ episome
• Applications:Preparation of ssDNA, construction of gene library, subcloning
• Efficiency:>5x10e7 cfu/ug
• Control Plasmid:(pUC19) 5ul (10e4ug/ul)
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.