85-6085-25　［取扱停止］ECOS™ 21 Competent Cells [BL21(DE3)] 5x100ul　583336

特徴

• This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
• Genotype:F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])
• Features:Lack of Lon and Omp proteases allows high-level protein expression and accumulationEasy gene induction
• Applications:IPTG induced gene expression in E. coliIdeal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectorsSubcloning
• Efficiency:>2x10e7 cfu/ug
• Control Plasmid:(pUC19) 5ul (10e-4ug/ul)
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.