85-6085-23　［取扱停止］ECOS™ 10B Competent Cells [DH10B] 10x100ul　583335

特徴

• DH10B is an MC1061 derivative. This strain was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.
• While DH10B has been classically reported to be galU galK, the preliminary genome sequence for DH10B indicates that DH10B is actually galE galK galU+. Genome sequence indicates that DH10B is actually deoR+. The complete genome sequence has been published.
• Features:Streptomycin resistantPrevent DNA methylationHigh transformation efficiency
• Applications:Suitable for genomic DNA cloningAllow large insertsCloning of methylated cytosine or adenine containing DNABlue/white screening
• Efficiency:>1x10e8 cfu/ug
• Control Plasmid:(pUC19) 5ul (10e-4ug/ul)
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.