Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences.
The T&A™ Cloning Kit contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3′-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency.
Features:Fast ligation, completed in only 5 minutesHigh transformation efficiencyMore accurate resultsAccept a wide range of inserts with different sizesTwo types of ligation buffers provided for your convenienceAllow blue/white screeningContain ampicillin marker for antibiotic selectionInclude M13 primer sites for convenient sequencing
Applications:Cloning of terminal 3’-dA nucleotides overhang PCR products up to 5 kbQuality ControlDNA concentration of T&A™ Cloning Vector is 25ng/ulThe absorbance ratio (A260/A280) is between 1.6-2.0The size of T&A™ Cloning Vector is about 2.7 kbThe colony number of background control is less than 50 when the transformation efficiency of competent cells is 1×10e8cfu/ug DNAThe colony number ratio of self-ligation control to positive control is less than 15%The colony number of the positive control is more than 500 when the transformation efficiency of competent cells is 5×10e8cfu/ug DNAThe ligation correctness with the control insert into T&A™ Cloning Vector is more than 87.5%
Kit Components:T&A™ Cloning Vector: 40ul (25ng/ul)Control Insert DNA: 10ul (10ng/ul)yT4 DNA Ligase:20ul (2U/ul)10X Ligation Buffer A: 50ul10X Ligation Buffer B: 50ulForward Primer (M13-F):50ul (10uM)Reverse Primer (M13-R):50ul (10uM)
