85-6084-10　CHST5, Recombinant, Mouse, aa27-395, His-Tag 10ug　497032

特徴

• CHST5, also known as carbohydrate sulfotransferase 5, is a Golgi- embedded enzyme that is found in T cells, B cells and intestinal epithelium. This sulfotransferase utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. It also mediates sulfation of keratan in cornea. It acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. It may also have activity toward Olinked sugars of mucin-type acceptors. There is no CHST6 found in the mouse genome it is possible that mouse CHST5 plays a similar biological role to the human CHST6.
• Recombinant protein corresponding to aa27-395 from mouse CHST5, fused to His-Tag at C-terminal, expressed in Baculovirus.
• Molecular Weight:~42.9kD
• Specific Activity:＞10,000 pmol/min/ug
• Unit Definition:Defined as the amount of enzyme that sulfate from PAPS to Nacetyl-D-glucosamine per minute at pH 7.5, at 37°C.
• Amino Acid Sequence:＜ADPEF＞SRQVP SSPAGLGERV HVLVLSSWRS GSSFVGQLFS QHPDVFYLME PAWHVWDTLS QGSAPALHMA VRDLIRSVFL CDMDVFDAYL PWRRNISDLF QWAVSRALCS PPVCEAFARG NISSEEVCKP LCATRPFGLA QEACSSYSHVVLKEVRFFNL QVLYPLLSDP ALNLRIVHLV RDPRAVLRSR EQTAKALARD NGIVLGTNGT WVEADPRLRV VNEVCRSHVR IAEAALHKPP PFLQDRYRLV RYEDLARDPL TVIRELYAFT GLGLTPQLQT WIHNITHGSG PGARREAFKT TSRDALSVSQ AWRHTLPFAK IRRVQELCGG ALQLLGYRSV HSELEQRDLS LDLLLPRGMD SFKWASSTEK QPES＜HHHHHH＞
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.