IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes.
The Interleukin 2 (IL-2) BioAssay™ ELISA Kit (Human) is a solid phase immunoassay specially designed to measure Human IL-2 concentrations in in cell culture supernates, serum, plasma, and other biological fluids.
Detection Range:15.6-1000pg/ml
Sensitivity:0.1pg/ml
Specificity:Recognizes natural and recombinant human IL-2, no detectable cross-reactivity with other relevant proteins.
Test Principle:The Interleukin 2 (IL-2) BioAssay™ ELISA Kit (Human) is a solid phase immunoassay specially designed to measure Human IL-2 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL-2. It contains E. coli-expressed recombinant Human IL-2. The detection antibody is a biotinylated goat polyclonal antibody specific for IL-2. The kit is analytically validated with ready to use reagents.
Storage and Stability:Store the unopened product at 2-8°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
