TNF alpha is produced by activated macrophages, monocytes, endothelial cells, fibroblast and both T and B lymphocytes. Production of ovine TNF alpha can be induced by a variety of factors, dependant upon cell type, and includes bacterial toxins, IL-1, PDGF, IFN beta, NGF, oncostatin M and viral infections. TNF alpha functions in the regulation of immune cells, is able to induce fever, apoptotic cell death, sepsis, cacheria, inflammation and to inhibit tumorigenesis and viral replication.
Source:Recombinant protein corresponding to aa78-234 from sheep Tumor Necrosis Factor (TNF) alpha expressed in Pichia pastoris
Molecular Weight:~17.2kD
Applications:Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% carrier protein. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：5ug
Source Antigen：Recombinant, Pichia pastoris
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a lyophilized powder in PBS, 10% trehalose. Reconstitute with 20ul sterile PBS, 0.1% carrier protein.
