Kubota’s Compete Serum-Free FL-HCC Spheroid Growth Medium is formulated for optimal growth of human FL-HCC Cancer Stem Cells in spheroids. Kubota’s FL-HCC Spheroid Growth medium contains glutamine but no antibiotics, a serum-free supplement and hyaluronans (non-sulphated high molecular weight glycosaminoglycans) then filtered to ensure sterility, frozen and maintained in -80°C ultralow freezer until ready for use. The medium is shipped on dry ice and when thawed, the medium is complete and ready to use for expansion of human FL-HCC cancer stem cells as spheroids.
Handling Kubota’s FL-HCC Spheroid Complete Growth Medium:1. Kubota’s FL-HCC Spheroid Growth medium is complete and readyto use:a. Store in -80°C ultralow freezer until ready for useb. For optimal performance place frozen bottle of media in 4°C refrigerator to thaw overnight. The next day it will be ready to use
c. You may also thaw in 37°C waterbathi. Place frozen bottle of media in waterbathii. Invert bottle every 5-10 minutes until only a small ice ball remains thenremove from the waterbathd. Do not leave media in 37°C waterbath for longer than 5 minutes as this mayresult in degradation of the growth factors, proteins and glutamine in the mediae. Store thawed media in 4°C refrigerator in the darkf. Use Kubota’s FL-HCC Spheroid Growth medium within one monthg. Do not re-freeze Kubota’s FL-HCC Spheroid Growth Medium
