Isolated from the whole brain of Harlan Sprague Dawley E14 rat brain tissue characterized by immunocytochemistry with neural stem cell antibodies and differentiation potential with antibodies for specific neural sub-types, astrocytes and oligodendrocytes. For best results should be cultured in low oxygen conditions.
Required Equipment:1. All procedures should be conducted inside a biological safety cabinet such as a NuAire Class II Type A2
2. The neural precursor cells can be maintained with an antibiotic if required in order to prevent bacterial contamination. All our lines have been tested with gentamycin (30ug/ml)
3. Incubator should be low oxygen capable such as the NuAire 4950 with the following settings:a. 6% CO2b. 2-4% O2c. 95% humidityd. 37°CNote: It is best to culture neural cells in low oxygen conditions for optimal growth of rNSCs and differentiation into neurons. Atmospheric oxygen incubators may be used but will result in slower growth of rNSCs and differentiated neural cultures with lower percentage of neurons and high percentage of astrocytes and oligodendrocytes.
Required Supplies:1. Neural StemCell Growth Mediuma. Contains Neural StemCell Growth Basal Medium and Neural StemCell Growth Supplement Pack containing the following components:Neural StemCell Growth Base Supplement (contains glutamine)LamininbFGFEGFProprietary Factor2. Neural Transition Medium to prepare NSCs for differentiationa. Contains Neural Transition Basal Medium and Neural Transition Supplement Pack containing:Neural Transition Base Supplement (contains glutamine)LamininbFGFEGF3. Neural Differentiation Medium for pan-neural differentiationa. Contains Neural Differentiation Basal Medium and Neural Differentiation Supplement Pack containing:Neural Differentiation Base Supplement (contains glutamine)LamininBDNFGDNF
Storage and Stability:Store the complete medium at 4°C in the dark until ready for use.
