Rat Neural Starter Kit with Hippocampal Neural Stem/Progenitor Cells
Required Equipment:1. All procedures should be conducted inside a biological safety cabinet such as a NuAire Class II Type A2
2. The neural precursor cells can be maintained with an antibiotic if required in order to prevent bacterial contamination. All our lines have been tested with gentamycin (30ug/ml)
3. Incubator should be low oxygen capable such as the NuAire 4950 with the following settings:a. 6% CO2b. 2-4% O2c. 95% humidityd. 37°CNote: It is best to culture neural cells in low oxygen conditions for optimal growth of rNSCs and differentiation into neurons. Atmospheric oxygen incubators may be used but will result in slower growth of rNSCs and differentiated neural cultures with lower percentage of neurons and high percentage of astrocytes and oligodendrocytes.
Required Supplies:1. Neural StemCell Growth Mediuma. Contains Neural StemCell Growth Basal Medium and Neural StemCell Growth Supplement Pack containing the following components:Neural StemCell Growth Base Supplement (contains glutamine)LamininbFGFEGFProprietary Factor2. Neural Transition Medium to prepare NSCs for differentiationa. Contains Neural Transition Basal Medium and Neural Transition Supplement Pack containing:Neural Transition Base Supplement (contains glutamine)LamininbFGFEGF3. Neural Differentiation Medium for pan-neural differentiationa. Contains Neural Differentiation Basal Medium and Neural Differentiation Supplement Pack containing:Neural Differentiation Base Supplement (contains glutamine)LamininBDNFGDNF
Kit Components:1X Bottle Neural Stem Cell Growth Media 1X Bottle Neural Transition Media1X Bottle Neural Differentiation1X Bottle Neural Freezing Media1X Bottle Trypsin1X Bottle Trypsin Inhibitor1X Vial of rat Hippocampal NSPCs
Storage and Stability:Storage components as noted on protocol.
