Cryopreserved Hepatocyte Recovery Medium (CRM) is formulated for the optimal recovery of adult and neonatal cryopreserved human hepatocytes. CRM is shipped frozen on dry ice and upon thawing is complete and ready to use for recovery of cryopreserved hepatocytes. Thaw 1-2 vials ofcryopreserved human hepatocytes per 50ml of CRM.
Materials Needed When Thawing Cryopreserved Human Hepatocytes:1. Cryopreserved Hepatocyte Recovery Mediuma. Can thaw 1-2 vials of hepatocytes into 50ml Recovery Mediab. Comes frozen and is ready for use upon thawing2. Cryopreserved Hepatocyte Plating Mediuma. Make according to the media protocol accompanying the medium3. Hepatocyte Culture Mediuma. Make according to the media protocol accompanying the medium4. Follow the Recovery and Culture of Cryopreserved Hepatocytes Methods
