Human/Human Hepatic Co-Culture Model Cryopreserved Kit
Cryopreserved adult and neonatal hepatocytes require special handling to ensure optimal viability following the freeze/thaw process. Please follow these methods closely to ensure the best performance of human hepatocytes in culture.
Kit Components:1x vial Cryopreserved Human Hepatocytes1x vial Cryopreserved Growth Arrested Human Liver Stromal Cells. 1x Bottle of Cryopreserved Hepatocyte Recovery Media1x Bottle of Hepatocyte Plating Media1x Bottle of Hepatocyte Culture Media
Required Equipment:1. All procedures should be conducted inside a biological safety cabinet such as a NuAire Class II Type A2
2. Incubator should be controlled for humidity, temperature and CO2 concentration such as a NuAire 4950 with the following settingsa. 6% CO2b. 95% humidityc. 37°CNote: It is best to fyrite incubators regularly to ensure appropriate gas concentration is maintained.
Required Supplies:1. Fresh or cryopreserved Human hepatocytes2. Cryopreserved growth arrested Human liver stromal cells3. Cryopreserved Hepatocyte Recovery Media4. Hepatocyte Plating Media5. Hepatocyte Culture Medium6. Collagen I coated plates 7. Liver biomatrix coated plates
Storage and Stability:Upon receipt of the cells, immediately transfer the vials into your liquid nitrogen freezer.
