Pieces of a human fibrolamellar hepatocellular carcinoma (FL-HCC) solid tumor that has been propagated in a mouse Patient Derived Xenograft (PDX) model and contains the DNAJB1-PRKACA fusion transcript.
Cryopreserved or fresh human FL-HCC Cancer Stem Cell Spheroids require special handling to ensure optimal viability and growth of fresh spheroids or following the freeze/thaw process. Please follow these methods closely to ensure the best performance of the spheroids
Human FL-HCC Cancer Stem Cell Spheroids Growth Methods:Required Equipment:1. All procedures should be conducted inside a biological safety cabinet such as a NuAire Class II Type A2
2. FL-HCC Cancer Stem Cell Spheroids can be maintained with an antibiotic if required in order to prevent bacterial contamination. Our cells have been tested with gentamycin (30ug/ml). 3. Incubator should be controlled for humidity, temperature and CO2 concentration such as a NuAire 4950 with the following settingsa. 6% CO2b. Normal O2c. 95% humidityd. 37°CNote: It is best to fyrite incubators regularly to ensure appropriate gas concentration is maintained.
Required Supplies:1. Cryopreserved human FL-HCC Cancer Stem Cell Spheroids2. Kubota’s FL-HCC Spheroid Growth Media3. FL-HCC Spheroid Dissociation Media4. Spheroid Wash Buffer5. FL-HCC Spheroid Freezing Media6. Petri dishes or flasksa. Falcon/Corning petri dishes:i. 35 x 10mm petri dishii. 60 x 15mm petri dish iii. 100 x 15mm petri dish iv. 15 x 150mm petri dishb. Nunc petri Dishes:i. 35 x 10mm petri dish ii. 60 x 15mm petri dishiii. 100 x 20 mm petri dish iv. 150 x 25mm petri dish c. Falcon/Corning Petri Flasks d. Suspension Culture Flasks:i. T25 suspension filter cap flaskii. T75 suspension filter cap flaskiii. T175 suspension filter cap flask 7. 70% ethanol8. Chlorine Bleach
Storage and Stability:Upon receipt of the package, immediately transfer the cryopreserved vials into your liquid nitrogen freezer and store until ready for use. For optimal performance of the cells, do not allow vials to warm where frost forms.
