The target protein TMPRSS2 is a cell surface protein mainly expressed by endothelial cells across the respiratory and digestive tracts. It participates in the cleavage of peptide bonds of proteins that have serine as the nucleophilic amino acid within the active site acts. Emerging evidence has shown that TMPRSS2 acts to activate the SARS-CoV-2 spike (S) protein and facilitate viral entry into host cells.
Partial recombinant protein corresponding to amino acid residues 106-493 from human transmembrane protease serine 2 (TMPRSS2) (R255Q), fused to 10X His-Tag at N-terminal, expressed in mammalian cells.
Enzyme Activity: Measured by its ability to cleave fluorogenic peptide substrate (Boc-Gln-Ala-Arg-AMC), the Km is 19.16uM.
Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：20ug
Source Antigen：Recombinant, Mammalian cells
Grade：Purified
Purity：≥90% (SDS-PAGE) Endotoxin: ≤1EU/ug (LAL)
Form：Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O.
