The BioSeparation™ Protein Marker/Ladder consists of 11 proteins that resolve into sharp, tight bands in the range of 10-175kD. This unique protein ladder allows you to monitor protein separation during electrophoresis, estimate molecular weight of the protein of interest, and evaluate western blot transfer efficiency. It also includes ~10, ~40 and ~90 kD reference bands coupled with blue dye that allow easy tracking and identification.
Applications:Designed for monitoring protein migration during SDS-PAGE, monitoring efficiency of protein transfer onto PVDF or nitrocellulose for Western Blotting, as well as sizing of proteins on SDS-PAGE and Western Blots.
Bands:11
Protein Range:10kD-175kD
Usage:Mix gently by pipetting up and down a few time, then directly load 5ul into well of gel.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
