85-6052-79　HpyF3I (DdeI), 10U/ul 500U　552380

特徴

• 5'C↓TNAG3' 3'GANT↑C5'
• HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer. Includes universal Tango buffer for double-digestions
• Concentration:~10U/ul
• Isoschizomers:BstDEI, DdeI
• Specifications:Methylation Sensitivity: Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitiveEnzyme: HpyF3I (DdeI)Compatible Buffer: 10x Buffer TangoSensitive to Heat Inactivation: YesOptimal Reaction Temperature: 37°CType IIS RE: No
• Unit Definition:One unit is defined as the amount of HpyF3I required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer.
• Storage Buffer:HpyF3I is supplied in: 10mM Tris-HCl, (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
• Thermal Inactivation:HpyF3I is inactivated by incubation at 65°C for 20 min.
• Applications:
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.