85-6052-77　FspBI (BfaI), 10u/ul 500u　552379

特徴

• 5'C↓T A G3' 3'GAT↑C5'
• FspBI (BfaI) restriction enzyme recognizes C^TAG sites and cuts best at 37°C in Restriction Enzyme Buffer A, 10X. Includes universal Restriction Enzyme Buffer A, 10X for double-digestions
• Unit Definition:One unit is defined as the amount of FspBI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer.
• Form:Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol
• Supplied with:R1625: Restriction Enzyme Buffer A, 10X:Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA
• Incubation Temperature:37ºC
• Thermal Inactivation:FspBl is inactivated by incubation at 65°C for 20 minutes
• Diluent Buffer:10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol
• Enzyme Properties:Methylation Effects:Dam, Never overlaps - no effectDcm: Never overlaps - no effectCpG: Never overlaps - no efectEcoKI: Never overlaps - no effectEcoBI: Never overlaps - no effect
• Stability during Prolonged Incubation: A minimum of 0.3units of FspBl is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
• Digestion of Agarose-embedded DNA: A minimum of 10 units of FspBl is required for complete digestion of 1ug of agarose-embedded lambda DNA in 4 hours.
• Compatible Ends:Csp6l, Ndel, Tru1l, Vspl
• Number of Recognition Sites in DNA:Lambda: 13PhiX174: 3pBR322: 5pUC57: 4pUC18/19: 4pTZ19R/U: 5M13mp18/19: 5
• Overdigestion Assay:No detectable change in the specific fragmentation pattern is observed after a 160-fold overdigestion with FspB (10u/ug lambda DNA x 16 hours).
• Ligation and Recleavage (L/R) Assay:The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.
• Labeled Oligonucleotide (LO) Assay:No detectable degradation of single-stranded or double-stranded labeled oligonucleotides occurred during incubation with 10 units of FspB for 4 hours.
• Storage and Stability:May be stored at 4°C. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.