85-6052-63　［取扱停止］RNaseOFF Ribonuclease Inhibitor 1.25ml　552376

特徴

• RNaseOFF Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B, and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitor. RNaseOFF is stable even under very low concentrations of DTT (<1mM), making it the best choice for ultimate RNA protection.
• Source:Recombinant, E. coli
• Form:Liquid
• Concentration:100ul:40U/ul (4000Units/100ul)1.25ml:50KU/1.25ml
• Unit Definition:One unit is defined as the amount of RNaseOFF Ribonuclease Inhibitor that is required to inhibit the activity of 5ng of RNase A by 50%.
• Protocol:Always keep RNaseOFF on ice during reaction set-up. It is recommended to set-up all RNA related or RNase-sensitive work under conditions where RNase contamination has been eliminated. The use of “clean” pipettors designated for PCR and aerosol-resistant barrier tips are recommended. RNaseOFF should be added to the reaction tube prior to the addition of other components (including the sample) to ensure RNase inhibitory activity. Prevent inactivation of RNaseOFF and release of active ribonuclease by avoiding temperatures greater than 50°C, and high concentrations of urea or other denaturing agents.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.