85-6051-47　SEPSECS cDNA 10ug　552278

特徴

• SEPSECS, also known as SLA/LP autoantigen, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis.
• Vector Description:This shuttle vector contains the complete ORF. It is inseted BamH I to Hind III. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• cDNA Size:1506bp
• Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
• 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
• Each tube contains approximately 10ug of lyophilized plasmid.
• Nucleotide Sequence:ATGAACCGCG AGAGCTTCGC GGCGGGAGAG CGGCTGGTGT CGCCGGCTTA CGTGCGGCAG GGCTGTGAGG CCCGCCGCTC GCATGAGCAC CTCATACGGC TGCTTCTGGA GAAGGGCAAG TGTCCAGAGA ATGGCTGGGA TGAAAGTACA CTTGAACTCT TTTTACATGA ACTTGCAATC ATGGACAGCA ACAATTTCTT AGGCAATTGT GGTGTGGGAG AAAGGGAAGG GAGAGTGGCA TCCGCACTGG TTGCTCGTCG TCATTACAGG TTCATTCATG GCATTGGACG ATCCGGTGAT ATTTCTGCTG TGCAACCAAA AGCTGCAGGC TCTAGCCTTT TGAACAAAAT TACCAATTCT TTGGTCCTGG ACATTATAAA GCTGGCTGGT GTCCATACAG TAGCCAACTG CTTTGTAGTT CCTATGGCAA CTGGTATGAG TCTAACTCTG TGTTTCTTAA CATTACGACA CAAAAGACCA AAGGCAAAGT ATATTATATG GCCACGAATA GACCAGAAGT CCTGCTTTAA ATCCATGATC ACTGCAGGTT TTGAGCCTGT GGTGATAGAA AATGTTTTGG AAGGTGACGA GCTGCGTACA GACCTGAAAG CAGTGGAGGC TAAAGTCCAG GAACTTGGGC CTGATTGCAT TCTGTGTATT CATTCTACTA CATCCTGTTT TGCTCCAAGG GTGCCTGATA GATTAGAAGA ACTGGCTGTG ATTTGTGCTA ATTATGACAT TCCACATATA GTTAATAATG CTTATGGAGT GCAGTCTTCA AAGTGTATGC ATCTCATTCA GCAGGGGGCT CGAGTTGGTA GAATAGATGC TTTTGTTCAG AGCTTGGACA AAAATTTTAT GGTTCCAGTA GGTGGTGCTA TAATTGCTGG CTTTAATGAT TCATTCATTC AGGAAATCAG CAAGATGTAT CCAGGAAGAG CTTCAGCTTC ACCTTCTTTA GATGTCCTTA TTACTTTATT GTCACTTGGA TCAAATGGCT ATAAGAAGCT ACTAAAAGAA AGAAAGGAAA TGTTTTCATA TTTGTCCAAC CAAATAAAGA AGTTGTCAGA AGCCTACAAT GAAAGACTGT TGCATACACC TCACAATCCC ATATCTTTAG CTATGACACT TAAAACACTA GATGAACACC GTGACAAAGC TGTCACTCAG CTTGGCTCGA TGCTTTTTAC CAGACAGGTT TCTGGAGCCA GGGTTGTGCC TCTTGGGTCC ATGCAAACTG TGAGTGGCTA TACTTTCAGA GGCTTTATGT CACATACAAA TAATTACCCT TGTGCTTACC TCAATGCTGC ATCAGCCATC GGAATGAAGA TGCAGGATGT GGACCTGTTC ATAAAGAGAC TTGACAGGTG TTTAAAGGCA GTAAGAAAAG AACGAAGTAA AGAGAGTGAT GACAATTATG ACAAAACTGA AGATGTGGAT ATTGAAGAAA TGGCTTTAAA ACTAGATAAT GTACTTCTTG ACACATACCA GGATGCTTCT TCATGA
• Translation Sequence:MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.