85-6049-70　NAPA cDNA 10ug　552109

特徴

• NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues.
• Vector Description:This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• cDNA Size:888bp
• Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
• 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
• Each tube contains approximately 10ug of lyophilized plasmid.
• Nucleotide Sequence:ATGGACAATT CCGGGAAGGA AGCGGAGGCG ATGGCGCTGT TGGCCGAGGC GGAGCGCAAA GTGAAGAACT CGCAGTCCTT CTTCTCTGGC CTCTTTGGAG GCTCATCCAA AATAGAGGAA GCATGCGAAA TCTACGCCAG AGCAGCAAAC ATGTTCAAAA TGGCCAAAAA CTGGAGTGCT GCTGGAAACG CGTTCTGCCA GGCTGCACAG CTGCACCTGC AGCTCCAGAG CAAGCACGAC GCAGCCACCT GCTTTGTGGA CGCTGGCAAC GCATTCAAGA AAGCCGACCC CCAAGAGGCC ATTAACTGTT TGATGCGAGC AATCGAGATC TACACAGACA TGGGCCGATT CACGATTGCG GCCAAGCACC ACATCTCCAT TGCTGAGATC TATGAGACAG AGTTGGTGGA CATCGAGAAG GCCATTGCCC ACTACGAGCA GTCTGCAGAC TACTACAAAG GCGAGGAGTC CAACAGCTCA GCCAACAAGT GTCTGCTGAA GGTGGCTGGT TACGCTGCGC TGCTGGAGCA GTATCAGAAG GCCATTGACA TCTACGAACA GGTGGGGACC AATGCCATGG ACAGCCCCCT CCTCAAGTAC AGCGCCAAAG ACTACTTCTT CAAGGCGGCC CTCTGCCACT TCTGCATCGA CATGCTCAAC GCCAAGCTGG CTGTCCAAAA GTATGAGGAG CTGTTCCCAG CTTTCTCTGA TTCCCGGGAA TGCAAGTTGA TGAAAAAATT GCTAGAGGCC CACGAGGAGC AGAATGTGGA CAGCTACACC GAGTCGGTGA AGGAATACGA CTCCATCTCC CGGCTGGACC AGTGGCTCAC CACCATGCTG CTGCGCATCA AGAAGACCAT CCAGGGCGAT GAGGAGGACC TGCGCTAA
• Translation Sequence:MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.