85-6048-68　HYLS1 cDNA 10ug　552021

特徴

• HYLS1 encodes a protein localized to the cytoplasm. Mutations in this gene are associated with hydrolethalus syndrome. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Vector Description:This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• cDNA Size:900bp
• Preparation before Usage:1. Centrifuge at 7000rpm for 1 minute
• 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA
• Each tube contains approximately 10ug of lyophilized plasmid.
• Nucleotide Sequence:ATGGAAGAACTTCTACCTGATGGACAAATATGGGCTAATATGGATCCAGAAGAACGAATGTTGGCAGCTGCTACAGCTTTTACCCACATCTGTGCAGGGCAGGGTGAAGGAGATGTCAGGAGAGAAGCCCAATCTATCCAATATGATCCCTACAGTAAAGCTTCAGTAGCCCCAGGGAAGCGACCTGCTCTTCCTGTGCAACTACAGTACCCACATGTAGAAAGTAATGTCCCTTCAGAAACAGTCTCTGAGGCCTCCCAAAGACTCCGAAAGCCAGTGATGAAGAGAAAGGTGCTGCGCAGAAAGCCAGATGGGGAAGTATTAGTAACAGATGAGTCGATTATCAGTGAATCAGAATCTGGTACAGAAAATGATCAGGATCTCTGGGACTTAAGACAAAGGCTGATGAATGTACAGTTCCAGGAAGACAAGGAATCTTCATTTGATGTTTCACAAAAATTTAACCTACCACATGAATACCAAGGAATTTCTCAAGATCAGCTCATTTGCTCTCTACAAAGAGAAGGAATGGGCTCTCCAGCTTACGAACAAGACCTGATTGTTGCCAGCAGACCCAAGTCCTTTATTCTCCCAAAGCTGGACCAGTTAAGCCGAAACCGGGGCAAGACAGACCGGGTAGCCCGGTATTTTGAGTACAAACGGGACTGGGACTCAATACGTTTACCTGGTGAAGATCATAGAAAGGAATTACGCTGGGGTGTCCGAGAGCAGATGCTTTGTCGAGCAGAACCCCAATCCAAACCTCAGCATATATATGTCCCAAACAATTATCTAGTACCAACAGAGAAGAAAAGGTCTGCACTCCGTTGGGGTGTTCGTTGTGACCTTGCAAATGGTGTCATACCCAGGAAGCTTCCCTTCCCTCTTTCTCCTTCTTAA
• Translation Sequence:MEELLPDGQI WANMDPEERM LAAATAFTHI CAGQGEGDVR REAQSIQYDP YSKASVAPGK RPALPVQLQY PHVESNVPSE TVSEASQRLR KPVMKRKVLR RKPDGEVLVT DESIISESES GTENDQDLWD LRQRLMNVQF QEDKESSFDV SQKFNLPHEY QGISQDQLIC SLQREGMGSP AYEQDLIVAS RPKSFILPKL DQLSRNRGKT DRVARYFEYK RDWDSIRLPG EDHRKELRWG VREQMLCRAE PQSKPQHIYV PNNYLVPTEK KRSALRWGVR CDLANGVIPR KLPFPLSPS
• Storage and Stability:Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.